Identification of two germ-tube-specific cell wall antigens of Candida albicans
نویسندگان
چکیده
منابع مشابه
New monoclonal antibody specific for Candida albicans germ tube.
Hydrophobic components of the germ tube of the dimorphic pathogenic fungus Candida albicans were used as immunogens to prepare monoclonal antibodies (MAbs). Among the resulting MAbs, one (MAb 16B1-F10) was shown by indirect immunofluorescence to be specific to the surface of the mycelium phase of the C. albicans and C. stellatoidea species. No labeling of any other genera and Candida species te...
متن کاملCharacterization of Candida albicans cell wall antigens with monoclonal antibodies.
The antigenic composition of Candida albicans is very complex. In order to study the antigenic relationship between blastoconidia and germ tubes of C. albicans, we produced several monoclonal antibodies and analyzed their reactivity against cell wall antigens either in intact cells or in cells treated with dithiothreitol. Overall, four types of reactivity were found. Monoclonal antibodies 3D9 a...
متن کاملSerum tube identification of Candida albicans.
The production of filaments (germ tubes) by cells of Candida albicans in serum tubes permits presumptive identification to be made within two to three hours. The proportion of yeast-like cells forming filaments is progressively decreased with increasing cell concentration. The test is effective over a comparatively wide range of temperatures and using different types and concentrations of sera.
متن کاملSecretion, interaction and assembly of two O-glycosylated cell wall antigens from Candida albicans.
The mechanisms of incorporation of two antigens have been determined using a monoclonal antibody (3A10) raised against the material released from the mycelial cell wall by zymolyase digestion and retained on a concanavalin A column. One of the hybridomas secreted an IgG that reacted with two bands in Western blots. Indirect immunofluorescence showed that the antigens were located on the surface...
متن کاملCandida albicans cell wall proteins.
The Candida albicans cell wall maintains the structural integrity of the organism in addition to providing a physical contact interface with the environment. The major components of the cell wall are fibrillar polysaccharides and proteins. The proteins of the cell wall are the focus of this review. Three classes of proteins are present in the candidal cell wall. One group of proteins attach to ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1986
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.54.3.864-868.1986